Research about the influence of acetic acid toward Fe, Cu and protein concentration in the leaves of water hyacinth has been carried out. Heavy metal concentrations were analyzed using atomic spectroscopy method after wet destruction and protein samples were analyzed using the Kjedahl method. Treatment of various concentrations of acetic acid were 0.5% (v / v), 1.0% (v / v) and 2.0% (v / v) as …