Long whiskers catfish (Mystus gulio) is fish species from the Bagridae family of the Mystus genus. Long whiskers catfish in Indonesia were found in Java, Kalimantan and Sumatra. This research aimed to determine the COI gene sequence of mitochondrial DNA, the genetic distance and phylogenetic of long whiskers catfish habitat. This research was carried out in December 2023-March 2024. Fish sample…