Sambiloto leaves (Andrographis paniculate (Burm. F) Wall ex. Ness) contain andrografolid and flavonoids as the main compounds that have potential as antibacterial. Nanoemulsion with a combination of tween 20 and PEG-400 was carried out to produce preparations that have better antibacterial effects and are thermodynamically stable. This study was conducted to determine the characteristics and st…