Matoa (Pometia pinnata J.R.& G.Forst) is a plant from the Sapindaceae family with high genetic variation due to natural cross-pollination. This study aims to analyze the genetic variation and phylogenetic relationships between Kungkil and several Matoa variants (Red Matoa, Yellow Matoa, and Green Matoa) based on Internal Transcribed Spacer (ITS) rDNA sequences. The methods used include DNA isol…