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UJI AKTIVITAS EKSTRAK DAUN KARAMUNTING (Rhodomyrtus tomentosa (Aiton) Hassk) TERHADAP BAKTERI Pseudomonas aeruginosa

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Background: Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that frequently causes nosocomial infections and exhibits strong biofilmforming ability as well as high resistance to various antibiotics, including carbapenems. The increasing incidence of MDR-P. aeruginosa highlights the need for alternative antimicrobial agents derived from natural sources. Karamunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk) contain secondary metabolites such as flavonoids, tannins, saponins, triterpenoids, phenols, and alkaloids that have been reported to possess antibacterial activity, making them potential candidates for natural antibacterial development against resistant bacteria. Methods: This research was a descriptive laboratory experimental study. Six primary treatment groups were tested at concentrations of 125 µg/mL, 250 µg/mL, 500 µg/mL, 1000 µg/mL, and 2000 µg/mL, along with a control group. Additional concentrations of 4000 µg/mL, 6000 µg/mL, 8000 µg/mL, and 10,000 µg/mL were also included. The Minimum Inhibitory Concentration (MIC) was assessed using the disc diffusion method and broth dilution, while the Minimum Bactericidal Concentration (MBC) was determined using the solid dilution method. All procedures were carried out in four replications based on the Federer formula. Results: The results showed that the MIC obtained through broth dilution was 500 µg/mL. However, no MBC value was identified. Phytochemical screening confirmed that the karamunting leaf extract contained alkaloids, flavonoids, triterpenoids, saponins, tannins, and phenols. Conclusion: Karamunting leaf extract contains several secondary metabolites capable of inhibiting bacterial growth; however, it is not sufficiently effective in exerting bactericidal activity against the Gram-negative bacterium Pseudomonas aeruginosa.

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Detail Information

Series Title

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Call Number

T1870652025

Publisher

Palembang : Prodi Pendidikan Kedokteran, Fakultas Kedokteran Universitas Sriwijaya., 2025

Collation

xviii, 106 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

-

Classification

615.320 7

Content Type

Text

Media Type

unmediated

Carrier Type

other (computer)

Edition

-

Subject(s)

Tumbuhan obat
Prodi Pendidikan Kedokteran

Specific Detail Info

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MI

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