Skripsi

PENGARUH NAPHTHALENE ACETIC ACID DALAM MEDIA MURASHIGE AND SKOOG TERHADAP INDUKSI KALUS DAUN DURIAN

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Durian is a key commodity with high economic value in South Sumatra, but the generative propagation of this plant still faces obstacles. This research aims to determine the effect of NAA (Naphthalene Acetic Acid) application in MS (Murashige and Skoog) medium on callus induction in durian leaf explants (Durio zibethinus Murr.). The research used a completely randomized design (CRD) with five levels of NAA concentration, namely, each repeated three times with each unit consisting of 10 culture bottles. The N0 = 0 ppm, N1 = 1 ppm, N2 = 2 ppm, N3 = 3 ppm, and N4 = 4 ppm research results showed that the N0 treatment produced 83% live explants with 16.7% contamination, N1 produced 13% callus growth with 36.7% contamination, while N2 produced 80% live explants, 20% callus growth, and 20% contamination. Treatment N3 had the highest contamination at 73% with 26.7% live explants, while N4 produced 100% live explants but only 6.7% callus growth. During the 8-week observation period, the highest callus formation occurred in treatment N2. This research concluded that low NAA concentrations in the range of 1–2 ppm are more optimal for inducing callus in durian leaf explants, while excessively high NAA concentrations inhibit callus formation and increase the risk of contamination. These results can be used as a reference in optimizing durian tissue culture media to accelerate vegetative propagation and support the development of tissue culture techniques in woody plants.

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Detail Information

Series Title

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Call Number

T1899612025

Publisher

Indralaya : Prodi Agronomi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya., 2025

Collation

xiii, 50 hlm.; ilus.; tab.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

634.043 607

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Jurusan Budidaya Pertanian
Prodi Agronomi
Jurusan Budidaya Pertanian--Budidaya Durian
Budidaya Durian

Specific Detail Info

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Statement of Responsibility

MI

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