Skripsi
EFEKTIVITAS MIKROBA DARI PEMBIAKAN BERSERI Pyricularia oryzae, KITIN DAN RIMPANG KUNYIT TERHADAP P. oryzae PATOGEN BLAS PADI
Rice blast disease is an important disease caused by the fungus P. oryzae, which can lead to significant yield losses. An alternative control method that can be used to control the attack of the pathogenic fungus is by using plant extracts. One type of plant that has a high ability to suppress the rate of blast development is using turmeric rhizome extract. Therefore, this research was conducted to evaluate and determine the effectiveness of microbes from serial propagation of P. oryzae, chitin, and turmeric rhizomes against P. oryzae, the pathogen of rice blast disease. This study used a split-plot design with the main plot having 2 levels of enrichment materials, the subplot having 4 levels of serial propagation cycles, and 3 replications. The main plot levels were P. oryzae + sugar enrichment material and sugar + chitin + P. oryzae. The subplot levels were 0, 1, 3, and 5 propagation cycles. The observation variables were colony growth inhibition, EC test measurement, and mycelium observation under a microscope. Colonies of P. oryzae on MEA medium treated with microbial cells from root exudate (M0), after serial propagation of turmeric rhizomes and the addition of P. oryzae mycelium (RKUPG), showed growth inhibition with ANOVA F(3,32) = 544.33 and p < 0.0001, which was significantly different. The highest inhibition was observed in S1(RKUPG), showing 83.3% inhibition. The S1-RKUPG treatment showed the highest EC (Electrical conductivity) value, which was significantly different from the other treatments. After the first stage, radiant breeding no longer provided a significant increase in EC value. The ANOVA results showed an F(4,34) value of 8.54, and p < 0.0001, which was significantly different. The treatment of radiant propagation with the addition of turmeric rhizomes, P. oryzae mycelium, and chitin powder (RKUPGK) on colony inhibition showed a tendency toward differences between treatments. There is no significant difference in EC across all cycles in the RKUPGK. Keywords : Blast, Antagonist, EC, Endophyte, Hypha
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