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EFEKTIVITAS ANTIMIKROBA EKSTRAK DAUN MIANA (Coleus atropurpureus) TERHADAP Staphylococcus aureus MELALUI UJI ANTIMIKROBA KUANTITATIF DAN ANALISIS MORFOLOGI SEL

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Staphylococcus aureus infection remains a global health problem due to the increasing emergence of antimicrobial resistance, highlighting the need for alternative antibacterial agents derived from natural products. This study aimed to evaluate the antimicrobial effectiveness of ethanol extract of miana leaves (Coleus atropurpureus) against Staphylococcus aureus ATCC 25923 and to identify its bioactive compounds. This research was conducted as an in vitro experimental study using a post-test only design. Identification of bioactive compounds was performed using Liquid Chromatography–High Resolution Mass Spectrometry (LC-HRMS). Antibacterial activity was evaluated through the disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), as well as analysis of bacterial cell morphological changes using Scanning Electron Microscopy (SEM). Amoxicillin–clavulanate was used as a positive control, while the solvent served as a negative control. Differences in inhibition zone diameters were analyzed using the Kruskal–Wallis test followed by Dunn’s post hoc test (p < 0.05). The ethanol extract of Coleus atropurpureus contained various secondary metabolites predominantly belonging to the flavonoid group (genistein, luteolin, cymaroside, and apigenin). The extract exhibited concentration-dependent antibacterial activity against Staphylococcus aureus and demonstrated bactericidal effects at certain concentrations. SEM observations revealed morphological alterations of bacterial cells, including uneven cell surfaces and cell shrinkage, indicating damage to the cell wall and membrane structures. The ethanol extract of Coleus atropurpureus shows antimicrobial activity against Staphylococcus aureus and has potential to be developed as an alternative antibacterial agent derived from natural products. Keywords: Coleus atropurpureus; Staphylococcus aureus; LC-HRMS; antibacterial activity; SEM

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Detail Information

Series Title

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Call Number

T1907952025

Publisher

Indralaya : Prodi Magister Ilmu Biomedik, Fakultas Kedokteran Universitas Sriwijaya., 2025

Collation

xviii, 114 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

615.32107

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

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Subject(s)

Prodi Magister Ilmu Biomedik
Coleus Atropurpureus

Specific Detail Info

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Statement of Responsibility

KA

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