Skripsi
INDUKSI RESISTENSI MENGGUNAKAN SENYAWA ASAM FOSFIT (K₃PO₃) SEBAGAI PENGENDALIAN PENYAKIT Ceratocystis PADA TANAMAN DUKU (Lansium domesticum Corr.) DI SUMATERA SELATAN
Ceratocystis fimbriata is an important pathogen in duku (Lansium domesticum Corr.) plants that causes stem cancer, tissue rot, and rapid plant death, thereby significantly impacting duku production in South Sumatra. This study aims to evaluate the effectiveness of phosphite acid (K₃PO₃) in inducing duku resistance through in vitro and in vivo testing. The study was conducted using a completely randomized design with five phosphite acid concentration treatments (P0 = 0 mg/L, P1 = 250 mg/L, P2 = 500 mg/L, P3 = 1000 mg/L, and P4 = 2000 mg/L) with ten replicates. In vitro tests were conducted on MEA media to observe the inhibition of Ceratocystis mycelium growth, while in vivo tests were conducted by injecting fungicide into the stems of duku seedlings before pathogen inoculation. The parameters observed included mycelium growth, symptoms of attack, percentage of mortality, intensity of attack, lesion length, chlorophyll (CHL), and leaf nitrogen content. The results of the analysis showed that the application of phosphite acid had a significant effect on mycelium growth in vitro, with P1 (250 mg/L) being the most effective treatment in suppressing colony area. In vivo testing showed that phosphite reduced the severity of the disease, as indicated by a decrease in the intensity of attacks and plant mortality, particularly in treatments P1 and P3. Although there was no significant difference in the length of lesions on the upper and lower parts of the plant between treatments, the pattern of symptoms indicated that tissue damage tended to be more limited in plants that received phosphite application. Physiological parameters indicate that changes in chlorophyll and nitrogen levels were insignificant in most observations, although some treatments showed a downward trend in the late stages of infection. Overall, phosphite acid effectively inhibited pathogen development and enhanced plant resistance, particularly at low to medium concentrations, which produced the most stable inhibition without causing additional physiological stress.