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PENGARUH KONSENTRASI ZAT PENGATUR TUMBUH 2,4-D DAN BAP SERTA JENIS MEDIA PADA INDUKSI KALUS EKSPLAN DAUN SEMANGKA

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Tissue culture is a plant propagation method that has the potential to solve the problems of conventional watermelon seedling propagation. This study aims to determine effects of growth regulators 2,4-D and BAP and types of media on the callus induction of watermelon leaf explant. The study was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Sriwijaya University, using a Complete Randomized Factorial Design (CRFD) with two factors: media type (100% MS medium and 50% MS medium and 50% coconut water) and growth regulator concentration (1 ppm 2,4-D and 1 ppm BAP, 2 ppm 2,4-D and 1 ppm BAP, and 3 ppm 2,4-D and 1 ppm BAP). The results showed that 100% MS medium was able to increase the percentage of explants forming callus by 83.33%, increase explant mass by 259.5 g, and increase the percentage of live explants to 100%. A concentration of 3 ppm 2,4-D and 1 ppm BAP growth regulators was able to accelerate the time for explants to form callus to 14.35 days after inoculation. The 50% MS medium and 50% coconut water mixture had an effect on the contamination rate of 26.66%. Keywords: Coconut water, watermelon leaves, callus, Murashige and Skoog

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Detail Information

Series Title

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Call Number

T1914852026

Publisher

Indralaya : Prodi Magister Ilmu Tanaman, Fakultas Pertanian Universitas Sriwijaya., 2026

Collation

vi, 53 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

581.072 407

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

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Subject(s)

Air kelapa
Prodi Magister Ilmu Tanaman

Specific Detail Info

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Statement of Responsibility

KA

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