Skripsi
EFEKTIVITAS MIKROBA DARI PEMBIAKAN BERSERI PYRICULARIA ORYZAE, KITIN DAN TANAMAN KENCUR (KAEMPFERIA GALANGA) TERHADAP PATOGEN BLAS PADI.
Rice (Oryza sativa) is the primary crop that produces rice and plays an important role in supporting Indonesia’s economy. One of the major diseases that frequently infects rice plants is blast disease, which is caused by the fungus Pyricularia oryzae. As a more environmentally friendly alternative, the use of microorganisms as biological agents can inhibit and suppress pathogen development sustainably. The microorganisms used in this study were obtained from the rhizosphere of kencur plants. Therefore, this research was conducted to examine the ability of the microbial community from kencur root exudates, derived from serial cultivation with P. oryzae and chitin, in suppressing the growth and development of the pathogen P. oryzae. This study employed a split-plot design with three replications. The main plot consisted of two enrichment treatments: Pyricularia + sugar, and Pyricularia + sugar + chitin. The subplot treatments consisted of 0, 1, 2, and 3 cultivation cycles. Observed variables included colony growth inhibition, electrical conductivity (EC), and microscopic observations of mycelia. P. oryzae cultured on MEA was treated (M0), then after serial cultivation with kencur plants and the addition of P. oryzae mycelia (TKEPG), or after the addition of P. oryzae mycelia and chitin powder (TKEPK), showed highly significant effects (ANOVA p < 0.0001). The highest inhibition occurred in the second cultivation cycle (S2-TKEPG and S2 TKEPK). For electrical conductivity (EC), the TKEPG treatment showed a significant effect (ANOVA p = 0.0002), while the TKEPK treatment also showed a significant effect (ANOVA p < 0.0001). Mycelial growth began to appear abnormal or showed impaired absorption of lactophenol stain. The mycelial cell walls experienced damage or lysis. Keywords: inhibition, electrical conductivity, colony, mycelium, rhizosphere
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