Skripsi
UJI VIABILITAS DAN PEMBIAKAN MASSAL BAKTERI ENTOMOPATOGEN DALAM BAHAN PEMBAWA DI RUMAH BAYANG
Bagworm infestations significantly reduce oil palm productivity, emphasizing the need for environmentally safe biological control strategies. Entomopathogenic bacteria represent a promising biocontrol agent, yet their field performance depends heavily on maintaining high viability within an appropriate carrier. This study evaluated the viability of entomopathogenic bacteria formulated in empty fruit bunches (EFB) and palm kernel cake (PKC), and developed a practical mass- multiplication method suitable for semi-field application. A Completely Randomized Design with two treatments and ten replications was used, while bacterial viability was assessed on days 7, 14, and 21 using the Total Plate Count method; resulting data were transformed to log CFU/g and analyzed by ANOVA. Both carrier materials maintained high bacterial viability (8.69–9.31 log CFU/g), with peak growth on day 14 and no significant differences between carriers or incubation periods (p > 0.05). Greenhouse application confirmed the pathogenicity of the bacteria against bagworm larvae, inducing typical infection symptoms and mortality. The findings demonstrate that EFB and PKC are effective organic carriers for entomopathogenic bacteria and have strong potential for developing cost efficient, environmentally friendly biopesticide formulations for oil palm pest management. Keyword: Entomopathogenic Bacteria, Viability, Carrier Material, Empty Fruit Bunches, Palm Kernel Cake, Bagworms.