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IDENTIFIKASI MOLEKULER BAKTERI ENTOMOPATOGEN YANG DITEMUKAN PADA ULAT KANTUNG DI PERKEBUNAN KELAPA SAWIT

Validation of metagenomic analysis results through molecular identification is important to ensure that the detected entomopathogenic bacteria truly have potential as biological control agents. The objective of this research is to molecularly identify entomopathogenic bacteria in bagworms based on metagenomic data to confirm bacterial species. Bacterial samples were collected at the Bacteriology Laboratory, Faculty of Agriculture, Sriwijaya University. The research method using DNA barcoding includes DNA extraction, Polymerase Chain Reaction (PCR), electrophoresis, and 16S rRNA gene sequencing. The effectiveness of the bacteria was tested against Spodoptera litura larvae. Phylogenetic analysis was performed using MEGA 11.0, DNA sequences were compared via BLAST against NCBI GenBank for similarity analysis, and the percentage of larval mortality was analyzed using ANOVA, followed by the Honest Significant Difference (HSD) test. The 1500 bp 16S rRNA gene was analyzed using BLAST, showing that the PP bacterial isolate was similar to Stenotrophomonas maltophilia, the MC isolate was similar to Mammaliicoccus sciuri, and the MP isolate was similar to Bacillus thuringiensis. It was concluded that molecular identification using single gene sequencing was effective in identifying entomopathogenic bacteria of bagworms and had the potential as biological control agents.

Availability
#
Central Library (REFERENCE) T1908382025
T190838
Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T1908382025
Publisher
Indralaya : Prodi Agroekoteknologi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya.,
Collation
xiii, 114 hlm.; 29 cm.
Language
Indonesia
ISBN/ISSN
-
Classification
579.575 07
Content Type
Text
Media Type
-
Carrier Type
-
Edition
-
Subject(s)
Prodi Agroekoteknologi
Ulat kantung — hama kelapa sawit
Specific Detail Info
-
Statement of Responsibility
Other version/related
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