Skripsi

IDENTIFIKASI MOLEKULER BAKTERI ENTOMOPATOGEN YANG DITEMUKAN PADA ULAT API DI PERKEBUNAN KELAPA SAWIT

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Oil palm is an oil-producing plant that is susceptible to pest attacks, especially from the nettle caterpillar. Pest control using pesticides will have a negative impact on the environment and health. Entomopathogenic bacteria are an alternative for more environmentally friendly pest control. To confirm the characteristics of the bacteria, molecular identification can be used. This study aims to determine the results of metagenomic analysis in accurately identifying entomopathogenic bacterial species in nettle caterpillar using multi-gene DNA sequencing techniques at the Biomolecular Laboratory, Department of Agricultural Cultivation, Faculty of Agriculture, Sriwijaya University. This research was conducted from September to December by extracting bacterial isolates and proceeding to the Polymerase Chain Reaction (PCR) stage. The data obtained were used to construct a phylogenetic tree using MEGA 11.0 software and analyzed using NCBI (National Center for Biotechnology Information). The results showed that both genes had the same species results in the Mammaliicoccus and Bacillus genera, with a higher level of gyrB gene specificity. The results of gyrB gene sequencing analysis had a more accurate molecular evolution rate than the 16S rRNA gene, meaning that the gyrB gene was more specific in distinguishing closely related bacterial species.

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Detail Information

Series Title

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Call Number

T1909242025

Publisher

Indralaya : Prodi Agroekoteknologi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya., 2025

Collation

xv, 45 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

579.307

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

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Subject(s)

Prodi Agroekoteknologi

Specific Detail Info

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Statement of Responsibility

KA

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