Skripsi
KETERTARIKAN TRICHOGRAMMA SP. PADA DAUN PAKCOI YANG DIAPLIKASIKAN SECARA TOPIKAL BEAUVERIA BASSIANA DAN DIINFESTASIKAN TELUR DAN LARVA PLUTELLA XYLOSTELLA (LEPIDOPTERA:YPONOMEUTIDAE)
Plutella xylostella is the major pest of crops from the Brassicaceae family, causing economic losses of up to USD 4–5 billion annually worldwide. This study aims to determine the attraction of parasitoids to volatile compounds as potential attractants for egg parasitoids of P. xylostella treated with the fungus Beauveria bassiana. The research will employ a Randomized Block Design (RBD) using three olfactometer screening tests with the following treatments: sprayed with B. bassiana (a), sprayed with B. bassiana and infested with eggs (b), sprayed with B. bassiana and infested with larval movement, and a control sprayed with PDB (d). The level of parasitoid attraction to each treatment including fungi, larval movement, and eggs was compared with the control. The induction of B. bassiana applied topically to pakchoi plants, especially when combined with the presence of P. xylostella eggs, produced the strongest effect in attracting the egg parasitoid Trichogramma sp. The highest percentage of attraction was recorded in the egg-infested treatment. Meanwhile, control plants were proven not to produce chemical compounds capable of attracting Trichogramma sp. The preference toward volatiles emitted by plants infested with P. xylostella eggs indicates parasitoid attraction.The topical application of B. bassiana on plant leaves functions not only as a biological control agent against insects but also as an endophytic agent capable of colonizing plant tissues. The results of this study conclude that the attraction response of Trichogramma sp. to plants sprayed with the entomopathogenic endophytic fungus B. bassiana and infested with P. xylostella eggs successfully attracted parasitoids, demonstrating its potential as an attractant for Trichogramma sp. Further field research is required to verify the effectiveness of the topical method spraying B. bassiana on plants as an attractant capable of drawing parasitoids to control P. xylostella.
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