Skripsi
EFEKTIVITAS INOKULASI JAMUR ENDOFIT ENTOMOPATOGEN MELALUI PERENDAMAN BENIH BRASSICA RAPA TERHADAP MORTALITAS PLUTELLA XYLOSTELLA (LEPIDOPTERA:YPONOMEUTIDAE)
Plutella xylostella (Lepidoptera: Plutellidae) is a major pest of Brassicaceae crops and is capable of infesting plants at any developmental stage, with severe infestations potentially leading to total yield loss. Biological control of P. xylostella can be implemented through the application of entomopathogenic fungal endophytes that inhabit Brassicaceae plant tissues. Therefore, this research was conducted to determine the effect of entomopathogenic fungal colonization on pak choi seeds through seed treatment on the mortality of P. xylostella as the main pest of pak choi plants. This research used Randomized Block Design (RBD) method to evaluate the pathogenicity of 16 entomopathogenic fungi isolates using a seed treatment concentration of 1 × 108 and control treatment using only sterile water. 16 isolates of entomopathogenic fungi were able to live endophytically in pak choi plant tissues. On the 14th day after planting, pak choi plants induced by entomopathogenic fungi after being tested for fungi colonization showed a colonization rate of up to 66%. Larvae of P. xylostella that consumed colonized leaves showed reduced feeding activity, indicated by lower body weight compared to larvae in the control group. ignificant larval mortality occurred between days 5 and 12, with the highest mortality recorded on day 12 from the Beauveria bassiana isolates JgCrJr and JgSPK. These fungi isolates are the most pathogenic for controlling P. xylostella.
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