Skripsi
ISOLASI BAKTERI DARI LARVA ULAT KANTONG DI TANAMAN KELAPA SAWIT (Elaeis guineensis Jacq.) DAN POTENSINYA SEBAGAI MUSUH ALAMI
Oil palm (Elaeis guineensis Jacq.) is an important global plantation crop, but its production is often reduced by bagworm larvae (Metisa plana, Mahasena corbetti, Clania tertia, and Pteroma pendula), which can decrease yields by up to 40%. Synthetic insecticides are commonly used for control but may cause environmental pollution, resistance, and pest outbreaks. Bioinsecticides based on entomopathogenic bacteria offer an environmentally friendly alternative. This study aimed to detect entomopathogenic bacteria in bagworm larvae, characterize their morphological and physiological traits, and evaluate their potential as biological control agents. Three bacterial isolates, MC (Mammalicoccus), MP (Bacillus), and PP (Stenotrophomonas), were obtained. Morphology and physiology were examined using standard methods, SEM, and molecular identification based on 16S rRNA. Bioassays were conducted on Spodoptera frugiperda larvae to assess pathogenicity. The results showed distinct characteristics for each isolate. MC consisted of Gram-positive cocci forming yellow, mucoid colonies; MP was Gram-positive rods forming spore-containing, milky-white dry colonies; PP was Gram-negative rods with irregular, shiny colonies. MP (Bacillus thuringiensis) caused the highest larval mortality (60%), followed by PP (Stenotrophomonas malthopilia, 47.5%) and MC (Mammalicoccus sciuri, 35%). In conclusion, MP (Bacillus thuringiensis) is the most promising biological control agent for S. frugiperda. Further field trials and safety assessments on non-target organisms are recommended to ensure effective and sustainable application.