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IDENTIFIKASI MOLEKULER ULAT API (LIMACODIDAE) PADA PERKEBUNAN KELAPA SAWIT MENGGUNAKAN MULTIGENETIK MARKER

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Visually identifying nettle caterpillars species is often difficult due to the similarity in shape between species. Therefore, the molecular identification approach using DNA barcoding methods, utilizing multigene mitochondrial markers such as COI, COII, and Cyt b, is expected to provide a more effective and scientific solution for specifically identifying pest species. The purpose of this study is to accurately identify nettle caterpillars species using multigene DNA sequencing. The research methods used in this study include collecting samples of nettle caterpillars from oil palm plantations. The DNA barcoding stages consist of DNA extraction, DNA amplification using the polymerase chain reaction (PCR) method, electrophoresis, sequencing of the COI, COII, and CYT B genes, and data analysis using MEGA 11.0 software to display the nettle caterpillar phylogenetic tree. BLAST analysis of the nettle caterpillar sample's Parasa genus COI and CYT B genes showed a close relationship and very likely the same genus as the genus Parasa. The Thosea sample's COI and COII genes showed a close relationship within the same genus, genus Thosea. The Thosea sample's CYT B gene showed a close relationship within the same family, family Limacodidae. The Setora sample's COI, COII, and CYT B genes showed a close relationship within the same genus, genus Setora. The Olona sample's COI and CYT B genes showed a close relationship within the same family, family Limacodidae.

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Detail Information

Series Title

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Call Number

T1910122025

Publisher

Indralaya : Prodi Agroekoteknologi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya., 2025

Collation

viii, 234 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

632.707

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

hama serangga
Prodi Agroekoteknologi

Specific Detail Info

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Statement of Responsibility

TUTI

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