Skripsi
EFEKTIVITAS MIKROBA DARI PEMBIAKAN BERSERI COLLETOTRICHUM SP., KITIN DAN RIMPANG KUNYIT TERHADAP PATOGEN ANTRAKNOSA CABAI
Chili pepper (Capcium annum L.) is one of the high-value vegetable crops in Indonesia. Anthracnose disease in chili peppers is an important disease caused by the fungal pathogen Colletotrichum sp, which leads to significant yield losses. This alternative disease control method that can be used to reduce attacks from this disease is by utilizing the natural plant turmeric rhizomes. Therefore, this research was conducted to determine the effectiveness of microbes resulting from serial propagation of Colletotrichum sp. combined with chitin and turmeric rhizomes in inhibiting the growth of chili anthracnose pathogens. This research used a split-plot design with the main plot having 2 levels of enrichment material and the subplot having 4 levels of serial propagation cycles, with 3 replications. The main plot levels were Colletotrichum sp + sugar and Colletotrichum sp + sugar + chitin. The subplot levels were 0, 1, 3, and 5 propagation cycles. This study observed colony growth inhibition, EC measurements, and microscopic observations of mycelium. Colonies of Colletotrichum sp that have passed testing show varied results. Analysis of variance (ANOVA) on the inhibition of colonies by microbial cell treatments (KuC) showed differences between treatments (p ANOVA = 0.01). Analysis of variance (ANOVA) on electrical conductivity (EC) values for KuC treatments showed a significant effect (p ANOVA = 0.0004). Analysis of variance (ANOVA) on colony inhibition by KuCK treatments showed differences between treatments (p ANOVA = 0.002), and analysis of variance (ANOVA) on electrical conductivity (EC) values showed a significant effect (p ANOVA = 0.0001). Mycelium testing showed different results for M0, KuC, and KuCK treatments; the mycelium was disrupted, preventing the blue color of lactophenol from being fully absorbed into the hyphal tissue. The antagonistic microbial cells obtained from serial dilution were able to inhibit colony growth, increase the EC value, and cause lysis of the pathogen
No other version available