Skripsi
ISOLASI BAKTERI DARI LARVA ULAT API DI TANAMAN KELAPA SAWIT (ELAEIS GUINEENSIS JACQ.) DAN POTENSINYA SEBAGAI MUSUH ALAMI
Oil palm is the largest plantation commodity in Indonesia. The oil palm industry continues to grow but faces various challenges, one of which is pest infestation. The main pest is the nettle caterpillar, which damages the leaves and can inhibit photosynthesis, ultimately reducing yield. Control of this pest can be carried out using environmentally friendly entomopathogenic bacteria. This study aimed to identify entomopathogenic bacteria associated with nettle caterpillar larvae on oil palm plants. The research was conducted from August to November 2025 using observation, exploration, and purposive sampling methods. Field observations identified three species of nettle caterpillar larvae: Setora nitens, Parasa lepida, and Thosea vetusta. Bacteria were isolated, purified, and characterized morphologically, physiologically, and molecularly using 16S rRNA analysis. Three bacterial isolates were successfully obtained from different host species: SN (Mammaliicoccus), PL (Bacillus), and TV (Bacillus). The isolates showed variations in colony characteristics but shared similar physiological traits as Gram-positive, endospore-forming bacteria. Microscopic and biochemical test results indicate that the isolate belongs to the spore-forming bacterial group and is not pathogenic to palnts. These findings indicate that the entomopathogenic bacteria found in nettle caterpillars, particularly Bacillus, have potential to be developed as environmentally friendly biocontrol agents for pest management. Keywords: Bacillus, bacteria, entomopathogen, Mammalicoccus, Spodoptera frugiperda.
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