Skripsi
EFEKTIFITAS MIKROBA DARI PEMBIAKAN BERSERI Pyricularia oryzae, KITIN, DAN TANAMAN KUNYIT (Curcuma longa) TERHADAP PATOGEN BLAS PADI
Blast disease caused by the fungus Pyricularia oryzae is one of the most important diseases affecting rice crops throughout the world and causes significant yield losses. This study involved turmeric plants that were inoculated with Pyricularia mycelium and supplemented with chitin powder. The study design used split plots with two levels of supplement in the main plots and four levels of serial cultivation in the subplots, with three replicates. The main plot levels were Pyricularia+Sugar (KuPG), and Pyricularia+Sugar+Chitin (KuPGK). The subplot levels were 1, 2, and 3. The observation variables were: colony growth inhibition, EC measurement, and mycelium observation under a microscope. P. oryzae colonies on MEA medium with microbial cells from root exudates (M0) or after serial propagation of turmeric plants on cocopeat supplemented with Pyricularia mycelium (KuPG) showed growth inhibition with ANOVA F(3,32) = 2.89, P>0.05 (not significant). The highest inhibition was observed in S1-KuPG (35.3%) and S2-KuPG (9%). Electrical conductivity (EC) was not significant (P=0.68), increasing significantly in S1-KuPG, but colony inhibition decreased in M0, S2-KuPGK, and S3-KuPGK. Serial cultivation with turmeric plants, Pyricularia mycelium, and chitin powder (KuPGK) showed colony inhibition without differences between treatments (ANOVA F(3,32)=0.51, P=0.68). The highest inhibition was in S1-KuPGK (40.5%) and M0 (28.6%). EC was not significant (P=0.68), increasing in S1-KuPGK, but the pattern was not consistent with colony inhibition; low inhibition treatments (control, M0, S2-KuPGK, S3-KuPGK) had lower or no different EC. All treatments except the control showed mycelium destruction, indicated by the unabsorbed Lactophenol cotton bluesolution.
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