Skripsi

UJI AKTIVITAS ANTIINFLAMASI DAN TOKSISITAS AKUT EKSTRAK ETANOL HERBA SIRIH CINA (Peperomia pellucida L.) PADA TIKUS WISTAR

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Synthetic anti-inflammatory drugs are associated with the risk of serious adverse effects, highlighting the need for safer alternatives. Pepper elder (Peperomia pellucida L.) has been reported to exhibit anti-inflammatory activity in male rats; however, studies in female rats and data regarding its safety remain limited. This study aimed to evaluate the anti-inflammatory activity in female Wistar rats and to assess the safety of the ethanolic extract of pepper elder herb through an acute toxicity test. Anti-inflammatory activity was evaluated using the rat paw edema method in 25 rats divided into five groups: positive control (diclofenac sodium 4.5 mg/kgBW), negative control (0.5% Na CMC), and three treatment groups receiving the extract at doses of 100, 200, and 400 mg/kgBW. Acute toxicity testing was conducted using the Acute Toxic Class Method, consisting of a preliminary test with graded doses of 300, 2000, and 5000 mg/kgBW followed by a main test. The results showed that the ethanolic extract of pepper elder herb exhibited significant anti- inflammatory activity, with the highest percentage of inflammation inhibition of 62.24% at a dose of 400 mg/kgBW and an ED₅₀ value of 257.5 mg/kgBW. In the acute toxicity test, administration of the highest dose of 5000 mg/kgBW caused no mortality and no significant differences in blood biochemical parameters (p>0.05). The mean biochemical parameters in the normal group were SGOT 111.33±14.29 U/L, SGPT 43.67±4.04 U/L, urea 39.33±12.88 mg/dL, and creatinine 0.55±0.01 mg/dL, while those in the treatment group were SGOT 130.3±9.29 U/L, SGPT 39.33±6.50 U/L, urea 40.3±6.40 mg/dL, and creatinine 0.62±0.04 mg/dL. Macroscopic and microscopic examinations of organs showed normal conditions. Based on OECD guideline 423, the LD₅₀ value of the extract was determined to be greater than 5000 mg/kgBW and classified as non-toxic. The ethanolic extract of pepper elder herb demonstrated anti-inflammatory activity in female Wistar rats and showed a favorable safety profile.

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Detail Information

Series Title

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Call Number

T1921942026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xi, 174 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

-

Subject(s)

toksisitas akut
Prodi Ilmu Farmasi

Specific Detail Info

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Statement of Responsibility

KA

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