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PENGARUH PEMBERIAN ESTRADIOL TERHADAP EKSPRESI BIOMARKER NEKROPTOSIS DAN PERADANGAN PADA TIKUS MODEL SEPSIS

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Introduction: Sepsis is a leading cause of morbidity and mortality in critically ill patients. While estradiol administration shows potential as a sepsis therapy, its impact on the necroptosis process remains unclear. This study aims to investigate the effect of estradiol administration on necroptosis and inflammation in a sepsis-induced Wistar rat model. Methods: This experimental study employed a post-test only control group design using 32 male Wistar rats divided into four groups: negative control (KN), positive control administered 100 mg/kg corn oil (KP), first treatment group receiving 50 µg/kg estradiol (K1), and second treatment group receiving 100 µg/kg estradiol (K2). TNF-α and IL-6 levels were measured using ELISA; RIPK1, RIPK3, and CREB expressions were assessed via spleen immunohistochemistry; and MLKL levels were analyzed using flowcytometry. Data analysis included ANOVA, Kruskal-Wallis, Mann-Whitney, Bonferroni Post Hoc, regression, and PLS-SEM structural modeling. Results: The 100 µg/kg estradiol group significantly decreased the expression of necroptosis biomarkers (RIPK1, RIPK3, MLKL) and inflammatory levels (TNF-α, IL-6), while increasing the expression of the anti-inflammatory biomarker (CREB) in sepsis model rats (p

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Detail Information

Series Title

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Call Number

T1894192025

Publisher

Indralaya : Prodi Sains Biomedis Program Doktor Fakultas Kedokteran Universitas Sriwijaya., 2025

Collation

xviii, 295 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

616.027 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Sains Biomedis Program Doktor
Kedokteran Eksperimental

Specific Detail Info

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Statement of Responsibility

TUTI

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