Skripsi
PEMANFAATAN JAMUR ENDOFIT ASAL DAUN TANAMAN KARET SEBAGAI BIOKONTROL PENYAKIT GUGUR DAUN (Pestalotiopsis thailandica) PADA TANAMAN KARET (Hevea brasiliensis)
An important disease in rubber plants is leaf fall caused by Pestalotiopsis sp., characterized by small spots that expand into necrotic areas, leading to continuous leaf drop and canopy thinning. Environmentally friendly control can be achieved by utilizing antagonistic endophytic biological agents. Endophytic fungi are capable of producing bioactive compounds and secondary metabolites that play roles in parasitism, mutualism, and antagonism. This study aims to determine the diversity of endophytic fungi in rubber plants and their potential as antagonistic agents for controlling leaf fall disease. The research consists of two stages: exploration of endophytic fungi and testing their antagonistic potential against the pathogen Pestalotiopsis thailandica using a Completely Randomized Design (CRD) with six treatments, including a control and five replications. The observed variables include inhibition percentage, spore density, and dry weight. The results showed that five isolates of endophytic fungi were successfully identified from the exploration of rubber plant leaves. Endophytic fungal isolates from the clones BOM 24, PB 260, and IRR 39 consisted of three genera: Penicillium, Aspergillus, and Nigrospora. The highest inhibition percentage of endophytic fungi against Pestalotiopsis thailandica from day 1 to 11 was found in treatment P5, reaching 82.64%. This treatment also showed the lowest spore density value (0.56) and the lowest mycelial dry biomass (0.04 g), corresponding to the high inhibition percentage of the endophytic fungi. The Aspergillus fungus showed strong potential in suppressing the growth of the pathogen P. thailandica in vitro tests.
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