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AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI TENTATIF SENYAWA PADA FRAKSI KOLOM EKSTRAK ETANOL RIMPANG DLINGO (Acorus calamus L.)

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The rhizome of Acorus calamus L. (Dlingo) is a plant known to contain various secondary metabolites with potential natural antioxidant properties. This study aimed to evaluate the antioxidant activity of the ethanolic extract of A. calamus L. rhizome, determine the fraction exhibiting the highest antioxidant activity based on IC₅₀ values obtained from the DPPH assay, and identify compounds present in the selected subfraction using LC–MS/MS analysis. Extraction was performed using the maceration method with ethanol. The extract was subsequently fractionated using gravity column chromatography with a stepwise elution system based on polarity differences. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method by measuring percentage inhibition as a preliminary screening parameter and calculating IC₅₀ values as the primary quantitative parameter. In addition, total phenolic content (TPC) was determined using the Folin–Ciocalteu method, while total flavonoid content (TFC) was analyzed using the AlCl₃ complexation method. The results showed that the ethanolic extract exhibited a TPC value of 30,30 ± 0,047 mg GAE/g and a TFC value of 1,54 ± 0,231 mg QE/g. The antioxidant activity of the ethanolic extract was indicated by an IC₅₀ value of 111,73 µg/mL, while fraction F3 and subfraction F3.1 exhibited IC₅₀ values of 42,40 µg/mL and 196,23 µg/mL, respectively. Fraction F3 demonstrated the strongest antioxidant activity. LC–MS/MS analysis of subfraction F3.1 revealed the presence of ferulic acid and 3,4-diethoxyphenylacetic acid, which belong to the phenolic group. The findings suggest that antioxidant activity does not necessarily increase with further purification, implying the possible presence of synergistic interactions among compounds within the fraction that contribute to the antioxidant activity of A. calamus L. rhizome. Keywords: Acorus calamus L., antioxidant activity, free radical scavenging, fractionation, liquid chromatography mass spectrometry

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Detail Information

Series Title

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Call Number

T1930922026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xix, 121 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Ilmu Farmasi
Ekstrak Etanol Rimpang Dlingo

Specific Detail Info

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Statement of Responsibility

MI

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