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AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI TENTATIF SENYAWA PADA FRAKSI KOLOM EKSTRAK ETANOL RIMPANG TEMU KUNCI (Boesenbergia rotunda (L). Mansf.)

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Free radicals are reactive molecules that can induce oxidative stress and contribute to the development of various degenerative diseases. The use of natural antioxidants derived from plants is considered a safer alternative to synthetic antioxidants. The rhizome of fingerroot (Boesenbergia rotunda (L.) Mansf.) is known to contain secondary metabolites with potential antioxidant activity; however, previous studies have largely been limited to the evaluation of crude extracts. This study highlights its novelty by tracing antioxidant activity down to the level of active fractions and subfractions and identifying their constituent compounds. The objectives of this study were to determine the antioxidant activity of the ethanolic extract of fingerroot rhizome, identify the most active fractions and subfractions, and tentatively identify compounds with potential antioxidant activity. Extraction was carried out by maceration using 96% ethanol, followed by fractionation and purification using column chromatography with thin-layer chromatography monitoring. Total flavonoid and total phenolic contents were determined by UV–Vis spectrophotometry, antioxidant activity was evaluated using the DPPH method expressed as IC₅₀ values, and compound identification was performed using LC–MS/MS. The results showed that the ethanolic extract contained total flavonoids of 10.51 ± 0.091 mg QE/g and total phenolics of 25.32 ± 0.065 mg GAE/g, with an IC₅₀ value of 61.64 µg/mL. Fraction F5 exhibited active antioxidant activity with an IC₅₀ value of 37.43 µg/mL, while subfraction F2 was identified as the most active fraction with an IC₅₀ value of 23.23 µg/mL. Compound identification indicated the presence of pinostrobin in the active subfraction. Stepwise fractionation to the subfraction level enhanced the antioxidant activity of the ethanolic extract of fingerroot rhizome.

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Detail Information

Series Title

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Call Number

T1931282026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xix, 103 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

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Subject(s)

Boesenbergia rotunda
Prodi Ilmu Farmasi

Specific Detail Info

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Statement of Responsibility

KA

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