Skripsi
KULTUR BOTRYOCOCCUS BRAUNII DENGAN PERSENTASE PUPUK PERTANIAN BERBEDA DALAM MEDIA LIMBAH CAIR BUDIDAYA IKAN GABUS
Botryococcus braunii is a colonial green microalga with high potential as a source of valuable biomass. However, its large scale production is constrained by high production costs. The utilization of snakehead fish culture wastewater supplemented with agricultural fertilizers provides a more economical and sustainable growth medium. This study aimed to determine the optimal percentage of agricultural fertilizer mixture for the growth, biochemical composition, and bioremediation capacity of B. braunii cultured in snakehead fish culture wastewater. The experiment employed a completely randomized design with five treatments and three replications, consisting of agricultural fertilizer mixture percentages of P0 (0%), P1 (25%), P2 (50%), P3 (75%), and P4 (100%). The results showed that different agricultural fertilizer mixture percentages significantly affected all observed parameters, with the best performance obtained at P3 (75%), which produced a maximum cell density of 2.34 × 10⁷ cells mL⁻¹, a specific growth rate of 27.65% day⁻¹, BOD₅ reduction of 85.71%, ammonia (NH3) reduction of 84.94%, as well as lipid, protein, and carotenoid contents of 44.22%, 37.43%, and 0.082% respectively. The results showed that the addition of 75% agricultural fertilizer was the optimal condition for culturing Botryococcus braunii in snakehead fish culture wastewater under the conditions of this study.