Skripsi

OPTIMASI EKSTRAK MIKROALGA Spirulina platensis DAN UJI AKTIVITAS ANTIOKSIDAN SECARA IN VITRO

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Optimization of the extraction process and testing of the antioxidant activity of Spirulina platensis microalgae extract is important for the development of natural microalgae-based antioxidant sources. Optimization was performed using multilevel factorial design 23 with 24 runs in Design Expert® 13 using three factors, namely solvent type (ethanol, acetone), temperature (25°C, 40°C), and extraction time (60, 90 minutes) with the Ultrasonic Assisted Extraction (UAE) method. Further identification by thin-layer chromatography (TLC) confirmed the presence of xanthophyll, chlorophyll, pheophytin, and carotenoids, as an indication of antioxidant activity. The extract was then tested for its phytochemical profile and showed alkaloid, flavonoid, terpenoid, and steroid/triterpenoid content. The responses observed were chlorophyll A, chlorophyll B, and carotenoid levels measured by UV-Vis spectrophotometry. Optimal conditions were obtained at a temperature of 25°C, a time of 90 minutes, and ethanol solvent with chlorophyll A content of 1480.667 µg/mL, chlorophyll B of 209.047 µg/mL, and carotenoids of 109.780 µg/mL. The antioxidant activity of the optimal extract was tested using the DPPH and FRAP methods at varying extract concentrations of 250, 125, 62.5, and 31.25 ppm. The IC₅₀ values obtained were 213.924 µg/mL (moderate category) for DPPH and 1411.618 µg/mL (very weak category) for FRAP, while vitamin C showed very strong activity. The difference in IC₅₀ values between the two methods reflects differences in testing mechanisms and sensitivity, but overall confirms the potential of Spirulina platensis as a source of natural pigment-rich materials with antioxidant activity. Keywords: antioxidants, carotenoid, chlorophyll, optimation extraction, Spirulina platensis

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Series Title

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Call Number

T1939602026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xiii, 72 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Ilmu Farmasi
Obat bahan alam

Specific Detail Info

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Statement of Responsibility

TUTI

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