Image of PENGARUH PEMBERIAN NAPHTALENE ACETIC ACID DAN BENZYL AMINO PURINE TERHADAP EKSPLAN DAUN KOPI ROBUSTA (Coffea canephora) PADA MEDIA MURASHIGE AND SKOOG
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PENGARUH PEMBERIAN NAPHTALENE ACETIC ACID DAN BENZYL AMINO PURINE TERHADAP EKSPLAN DAUN KOPI ROBUSTA (Coffea canephora) PADA MEDIA MURASHIGE AND SKOOG

Robusta coffee can grow well in areas with an average annual temperature between 22ºC and 26ºC. This study aims to determine the effect and effectiveness of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine) on Robusta coffee leaf explants (Coffea canephora) using MS (Murashige and Skoog) medium. This study was conducted using a combination of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine). The NAA concentration used consisted of two levels, namely 0.5 ppm (N1) and 1 ppm (N2), while the BAP concentration consisted of three levels, namely 0 ppm (B0), 1 ppm (B1), and 2 ppm (B2). The results showed that treatment with 0.5 ppm NAA + 2 ppm BAP was the most effective in maintaining explant viability for 8 weeks after inoculation. This treatment also showed the best ability to induce callus formation, with a percentage of 56.3%. However, the best callus morphology quality was obtained in the treatment with 1 ppm NAA + 2 ppm BAP, which was characterized by a fresh green color and compact callus texture. This treatment also showed the lowest level of contamination. The results of this study can be used as a reference in determining the appropriate combination of NAA and BAP in robusta coffee tissue culture, particularly to achieve a balance between explant viability, callus formation, and callus morphological quality.

Availability
#
Central Library (Reference) T1935172026
T193517
Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T1935172026
Publisher
Indralaya : Prodi Agronomi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya.,
Collation
xviii, 39 hlm.; ilus.; 29 cm
Language
Indonesia
ISBN/ISSN
-
Classification
633.730 7
Content Type
Text
Media Type
unmediated
Carrier Type
-
Edition
-
Subject(s)
kopi robusta
Prodi Agronomi
Specific Detail Info
-
Statement of Responsibility
Other version/related
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