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UJI AKTIVITAS ANTIOKSIDAN DAN ANALISIS KANDUNGAN KIMIA DARI FRAKSI AKTIF BATANG LENGLENGAN (Leucas Lavandulifolia)

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ABSTRACT : Lenglengan (Leucas lavandulifolia) belongs to the Lamiaceae family. L. lavandulifolia is traditionally used in the treatment of headaches, migraines, rheumatism, and diabetes. Information on the activity and chemical content of L. lavandulifolia stems is still limited. The purpose of this study was to determine the antioxidant activity and chemical content of the most active column fraction. This study was conducted using the maceration method with solvents of increasing polarity (n-hexane, ethyl acetate, and methanol), followed by determination of total phenolic and flavonoid content and testing of antioxidant activity using the DPPH (2,2-difenil-1-pikrilhidrazil) method. The most active extract was separated by gravity column chromatography. The eluate was analyzed using TLC (Thin Layer Cromatography), and spots with similar patterns were combined into one fraction. Each fraction was tested for antioxidant activity, and the most active fraction was identified for its chemical content using GC-MS (Gas Cromatography-Mass Spektrometry).. The results showed that the highest phenolic content was found in the methanol extract at 17.38 mgGAE (Gallic Acid Equivalent)/g, while the highest flavonoid content was found in the ethyl acetate extract at 3.14 mgQE (Quarsetin Equivalent)/g. The DPPH test showed that the methanol extract was the most active with an IC50 value of 162.67 mg/L. The separation of the methanol fraction yielded 8 fractions, and the highest antioxidant activity was shown by fraction 8 from the column with an IC50 value of 119.80 mg/L. GC-MS analysis of fraction 8 showed the main components to be phenol, 2,4-bis(1,1-dimethylethyl) (9.48%) and diisooctyl phthalate (48.04%). The IC50 data for fraction 8 of L. lavandulifolia stem methanol has the potential to be developed as a source of antioxidant compounds.

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Detail Information

Series Title

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Call Number

T1941052026

Publisher

Indralaya : Prodi Ilmu Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xv, 79 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Tanaman obat
Prodi Ilmu Kimia

Specific Detail Info

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Statement of Responsibility

TUTI

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