Skripsi

EFEKTIVITAS KOMBINASI NAPHTHALENE ACETIC ACID DAN BENZYL AMINO PURINE TERHADAP EKSPLAN DAUN KOPI ROBUSTA (COFFEA CANEPHORA) PADA WOODY PLANT MEDIUM

---

Robusta coffee (Coffea canephora) is one of Indonesia's important plantation commodities, the propagation of which still faces obstacles when done conventionally. One effort to overcome this problem is through tissue culture techniques. This study aims to determine the effectiveness of the combination of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) concentrations on the growth of Robusta coffee leaf explants in Woody Plant Medium (WPM). This study used a Completely Randomized Factorial Design (CRFD) with a combination of growth regulators consisting of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine). The NAA concentration used consisted of two levels, namely 0.5 ppm (N1) and 1 ppm (N2), while the BAP concentration consisted of three levels, namely 0 ppm (B0), 1 ppm (B1), and 2 ppm (B2). There were six combination treatments obtained, namely (N1B0 = 0.5 ppm (N1) + 0 ppm (B0), N1B1 = 0.5 ppm (N1) + 1 ppm (B1), N1B2 = 0.5 ppm (N1) + 2 ppm (B2), N2B0 = 1 ppm (N2) + 0 ppm (B0), N2B1 = 1 ppm (N2) + 1 ppm (B1), N2B2 = 1 ppm (N2) + 2 ppm (B2)). Each treatment consisted of 30 culture bottles, so the total number of bottles used in this study was 180 bottles. The results of this study indicated that the combination of NAA and BAP produces different growth responses in Robusta coffee leaf explants. The N2B1 treatment (1 ppm NAA + 1 ppm BAP) showed the best results with the highest percentage of callus formation, a relatively stable percentage of live explants until the end of observation, and lower levels of contamination and browning compared to other treatments. Thus, the combination of NAA 1 ppm and BAP 1 ppm is the most effective treatment to support the growth of robusta coffee leaf explants on WPM medium.

---

Availability

Detail Information

Series Title

-

Call Number

T1935012026

Publisher

Indralaya : Prodi Agroekoteknologi, Jurusan Budidaya Pertanian, Fakultas Pertanian Universitas Sriwijaya., 2026

Collation

xiii, 42 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

-

Classification

633.707

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

-

Subject(s)

Prodi Agroekoteknologi
Sitokinin

Specific Detail Info

-

Statement of Responsibility

KA

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment