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OPTIMASI FORMULA DAN UJI ANTIOKSIDAN NANOSUSPENSI EKSTRAK KULIT BAWANG MERAH (Allium cepa L.) SECARA IN VITRO

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Red onion peels (Allium cepa L.) contain flavonoid, phenolic, and steroid compounds that serve as potential antioxidants. This study aimed to optimize the concentration of polymers and crosslinking agents in a nanosuspension formulation of red onion peel extract prepared using the ionic gelation method. Optimization was conducted using a 22 Factorial Design in Design-Expert 13® software, evaluating two factors: variations in the polymer ratio of chitosan:sodium alginate (30:15 and 15:15) and variations in calcium chloride volume (40 µL and 100 µL). The responses measured included particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency (%EE). The analysis results indicated that the optimum formula was obtained at a polymer ratio of 30:15 with the addition of 40 µL of CaCl₂. Characterization of this optimum formula resulted in a particle size of 119.06 ± 3.42 nm (n=3), a PDI of 0.4442 ± 0.01(n=3), a zeta potential of -27.74 ± 0.01 mV (n=3), and an %EE of 82.82 ± 0.09%(n=3). Verification results showed that all parameters were within the 95% Confidence Interval (CI) and 95% Prediction Interval (PI) ranges, while the one-sample t-test indicated no significant difference between predicted and actual values (p>0.05). Antioxidant activity testing using the DPPH method showed that the nanosuspension had strong antioxidant activity with an IC₅₀ value of 69.82 ± 0.52 (n=3) ppm. Stability testing using the cycling test method showed that the formulation remained physically stable without phase separation during storage. Keyword(s): Allium cepa L., antioxidant, formulation optimization, nanosuspension.

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Detail Information

Series Title

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Call Number

T2004642026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xx, 120 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Ilmu Farmasi
Ekstrak Kulit Bawang Merah

Specific Detail Info

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MI

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