Skripsi
EVALUASI JARAK GENETIK DAN KARAKTER AGRONOMI BEBERAPA VARIETAS PADI LOKAL (ORYZA SATIVA L.) DI LAHAN RAWA LEBAK MELALUI RAPD MARKER
Local rice varieties (Oryza sativa L.) cultivated in the swampy lowlands of South Sumatra possess specific adaptation potential, yet information regarding their genetic diversity remains limited. This study aimed to evaluate genetic distance using RAPD molecular markers and to select the agronomic potential of several local swamp rice varieties. The research was conducted using a Completely Randomized Design (CRD) on 9 local varieties (Bone 1 , Bone 2, Bone 3, Koneng 1, Pegagan 1, Pegagan 2, Petek, Siputih 1, Siputih 2) and 1 national superior variety (Inpari 32). Molecular analysis was performed using the PCR-RAPD technique with four primers (OPA 10, OPA 13, OPB 07, OPC 02), while data were analyzed using the Jaccard coefficient method and ANOVA for agronomic characters. The results showed that the four primers successfully amplified a total of 22 loci, with primer OPA 13 having the highest Polymorphism Information Content (PIC) value of 0.366. Cluster analysis divided the varieties into two main groups; the Pegagan 2 variety was identified as an outgroup with the lowest similarity coefficient (0.76), whereas Inpari 32 and Siputih 2 had the closest genetic relationship (0.97). Agronomic evaluation showed a significant effect on the parameters of stem diameter and flowering age, as well as a significant effect on grain yield, fresh stem weight, and dry stem weight. The Inpari 32 variety was recorded as the earliest maturing (95.7 DAP) with the highest grain yield, while Pegagan 2 had the tallest plant stature but the lowest yield. Keywords: agronomy, genetic diversity, local rice, RAPD, swampy lowlands
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