Skripsi

FORMULASI DAN UJI AKTIVITAS ANTIHIPERLIPIDEMIA NANOSUSPENSI EKSTRAK KULIT BAWANG MERAH (Allium Cepa L.) DENGAN VARIASI KITOSAN

---

Hyperlipidemia is one of the main risk factors for cardiovascular disease. Shallot (Allium cepa L.) peel extract contains flavonoids, particularly quercetin, which has potential antihyperlipidemic activity; however, it is limited by low bioavailability. This study aimed to formulate a chitosan–sodium alginate–based nanosuspension of shallot peel extract and to evaluate its physical characteristics and antihyperlipidemic activity in vivo. The nanosuspension was prepared using the ionic gelation method with varying concentrations of chitosan. Hyperlipidemia was induced by administering propylthiouracil (PTU) and a high-fat diet for 30 days, followed by 14 days of treatment. Blood lipid profiles were measured using enzymatic colorimetric methods: CHOD-PAP for total cholesterol, GPO-PAP for triglycerides, the precipitation method for HDL, and the indirect method for LDL. Initial evaluation was conducted on encapsulation efficiency to determine the optimal formulation. The results showed that the formulation containing 0.1% chitosan exhibited the highest encapsulation efficiency (97.764%) and was therefore selected as the best formula. Physical characterization of the selected formula revealed an average particle size of 252.0 ± 41.70 nm, a polydispersity index (PDI) of 0.36 ± 0.04, and a zeta potential of −15.46 ± 2.36 mV. Stability testing using a 12-day cycling test showed no changes in color, odor, or sediment formation. A decrease in pH of 8.78% and a reduction in encapsulation efficiency of 4.46% from the initial condition still indicated acceptable physical stability of the formulation. The antihyperlipidemic activity test demonstrated that the nanosuspension of shallot peel extract reduced total cholesterol by 51.73%, triglycerides by 42.35%, and LDL by 60.13%, while increasing HDL levels by 62.70%. The effectiveness of the nanosuspension was significantly better than that of the shallot peel extract alone (p < 0.05). Macroscopic observation of the liver showed greater improvement in liver condition in the nanosuspension group compared to the negative control group. These findings indicate that the nanosuspension of shallot peel extract possesses antihyperlipidemic activity. Keywords: antihyperlipidemic, chitosan, ionic gelation, nanosuspension, red onion peel

---

Availability

Detail Information

Series Title

-

Call Number

T1956142026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xvii, 245 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

-

Classification

615.321 07

Content Type

Text

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Prodi Ilmu Farmasi
Nanosuspensi

Specific Detail Info

-

Statement of Responsibility

TUTI

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment