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EFEK ANTIOKSIDAN THYMOQUINONE PADA TIKUS PUTIH (RATTUS NORVEGICUS) MODEL FIBROSIS GINJAL: KAJIAN TERHADAP KADAR MALONDIALDEHYDE DAN EKSPRESI GLUTATION PEROKSIDASE

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Introduction Chronic Kidney Disease (CKD) is a progressive condition characterized by structural and functional impairment of the kidneys, with fibrosis as a key mechanism that worsens the disease through excessive extracellular matrix accumulation. Oxidative stress plays a central role in this process, indicated by increased reactive oxygen species (ROS), elevated malondialdehyde (MDA) levels, and reduced antioxidant activity such as Glutathione Peroxidase (GPx). Thymoquinone (TQ), the active compound of Nigella sativa, possesses antioxidant and antifibrotic potential. This study aimed to evaluate the effects of TQ on MDA levels and GPx expression in a rat model of kidney fibrosis induced by Unilateral Ureteral Obstruction (UUO). Methods This experimental study used a post-test only with control group design to assess the antioxidant effects of thymoquinone (TQ) on MDA levels and GPx expression in white rats with kidney fibrosis. Thirty male Rattus norvegicus aged ±2 months and weighing 200–250 g were divided into six groups: normal, negative control, and three TQ treatment groups (5, 10, and 20 mg/kgBW). Kidney fibrosis was induced using the UUO method. Treatments were administered for 14 days. Blood samples and kidney tissues were analyzed for MDA, urea, creatinine levels, and GPx expression using spectrophotometry and RT-PCR. Data were analyzed using SPSS with a significance level of p0,05). The highest malondialdehyde (MDA) levels were observed in the UUO negative control group and the lowest in the 20 mg/kgBW TQ group, with significant differences in post hoc testing. Urea and creatinine levels increased significantly in the UUO group, while higher doses of TQ produced marked reductions. GPx expression was highest in the healthy group and lowest in the negative control, with significant increases in the 20 mg/kgBW TQ group. Tubulointerstitial injury scores and positively stained areas were reduced in the treatment groups. There was a positive correlation between MDA and kidney injury, and a negative correlation between GPx and injury severity. Conclusion Thymoquinone (TQ) demonstrates antioxidant effects in a rat model of kidney fibrosis with significantly increasing GPx expression and reducing MDA levels —particularly at 20 mg/kgBW. Keywords: Thymoquinone; Kidney fibrosis; Oxidative stress; Malondialdehyde (MDA); Glutathione Peroxidase (GPx); Unilateral Ureteral Obstruction (UUO); Rattus norvegicus.

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Series Title

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Call Number

T1962162026

Publisher

Indralaya : Prodi Kedokteran, Fakultas Kedokteran Universitas Sriwijaya., 2026

Collation

xxii, 174 hlm.; ilus.; tab, 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

616.610 7

Content Type

Text

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Prodi Kedokteran
Penyakit ginjal.

Specific Detail Info

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Statement of Responsibility

TUTI

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