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UJI AKTIVITAS IMUNOSTIMULAN EKSTRAK ETANOL DAUN KIRINYUH (Chromolaena Odorata L.) TERHADAP TIKUS PUTIH JANTAN GALUR WISTAR DENGAN METODE Carbon Clearance

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Kirinyuh leaves (Chromolaena odorata L.) belong to the Asteraceae family and are known to contain various bioactive compounds with potential immunostimulant activity. This study aimed to evaluate the immunostimulatory activity of the ethanol extract of kirinyuh leaves in male Wistar rats ind uced with carbon using the carbon clearance method. Extraction was carried out by maceration using ethanol as the solvent and yielded an extract with a percentage yield of 18.2%. Phytochemical screening indicated the presence of flavonoids, phenolics, tannins, saponins, alkaloids, terpenoids, and steroids. The experimental animals were divided into a normal group, negative control (0.5% Na CMC), positive control, and treatment groups receiving doses of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. The observed parameters included blood phagocytic activity, spleen organ weight, and the number of leukocytes and lymphocytes. Data were analyzed using the One-Way ANOVA test followed by a post hoc test. The results showed that variations in extract dose significantly affected the increase in phagocytic activity. Based on the analysis, the dose of 400 mg/kgBW produced the highest increase in activity, indicating stimulation of the nonspecific immune system through activation of phagocytic cells and proliferation of immune cells in the spleen. Administration of the extract also influenced spleen weight as well as leukocyte and lymphocyte counts; however, the observed changes remained within the normal physiological range. Overall, the ethanol extract of kirinyuh leaves demonstrated immunostimulant activity by enhancing the nonspecific immune response through the activation of phagocytic cells and stimulation of the spleen. Keywords : carbon clearance, chromolaena odorata, phagocytosis, immunomodulator, immunostimulant, leukocytes, lymphocytes, spleen, and wistar rats.

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Detail Information

Series Title

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Call Number

T1961042026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

x, 98 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

-

Subject(s)

Prodi Ilmu Farmasi
Daun Kirinyuh

Specific Detail Info

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Statement of Responsibility

KA

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