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OPTIMASI PRODUKSI GULA PEREDUKSI MELALUI HIDROLISIS ASAM DENGAN VARIASI KONSENTRASI HCl PADA BIOMASSA Porphyridium cruentum HASIL KULTUR MANDIRI

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Lucky Adiguna, 080151382227111. Optimization of Reducing Sugar Production through Acid Hydrolysis with Variations in HCl Concentration on Porphyridium cruentum Biomass from Independent Cultures (Supervisors : Dr. Riris Aryawati, S.T., M.Si and Dr. Muhammad Hendri, S.T., M.Si.) The increasing demand for renewable energy has encouraged the utilization of microalgae as a sustainable feedstock for third-generation bioethanol production. Porphyridium cruentum possesses a high carbohydrate content and an exceptionally low lignin content, making it a promising biomass source for bioethanol production. This study aimed to analyze the lignocellulosic composition and optimize reducing sugar production from acir hydrolysis of P. cruentum biomass using different concentrations of hydrochloric acid (HCl), selected for its effectiveness and ease of control during the hydrolysisi process. The research was conducted through a laboratory-based experimental approach using P. cruentum biomass obtained from independently cultivated cultures. The microalgae were grown through an upscalling cultivation method using sterile synthetic seawater. The advanced-scale culture (final harvesting stage) achieved at highest cell density of 1,62 x 107 cells/mL on day 26 and produced 272 g of dry biomass. Lignocellulosic analysis revealed cellulose, hemicellulose, and lignin contents of 15,86%, 14,37%, and 0,18% respectively. Acid hydrolysis was performed using HCl concentrations of 1%, 2%, and 3%, while reducing sugar content was determined using the 3,5-dinitrosalicylic acid (DNS) method. The results demonstrated that HCl concentration had a highly significant effect on reducing sugar production, with the 3% HCl treatment yielding the highest reducing sugar concentration of 11,32 mg/mL with a total sugar conttent of 7,1. This study indicates that P. cruentum requires further optimization and development as a sustainable bioethanol feedstock. Keywords: Bioethanol, Porphyridium cruentum, reducing sugar, stepwise cultivation, synthetic seawater

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Series Title

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Call Number

T2020422026

Publisher

Indralaya : Prodi Ilmu Kelautan, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xiii, 84 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

660.607

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Ilmu Kelautan
Teknik Biokimia--Pruduksi Gula Pereduksi

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MI

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