Skripsi

UJI ANTIDIABETES FRAKSI ETIL ASETAT DAUN PUCUK MERAH (Syzygium myrtifolium Walp.) TERHADAP TIKUS JANTAN TERINDUKSI DIET TINGGI LEMAK-FRUKTOSA DAN STREPTOZOTOSIN

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Type 2 diabetes mellitus is a metabolic disorder characterized by hyperglycemia due to insulin resistance and impaired pancreatic β-cell function. Red shoot leaves (Syzygium myrtifolium Walp.) contain active compounds such as phenolics and flavonoids with antidiabetic potential. This study aims to determine the compound groups of the ethyl acetate fraction of red shoot leaves, the effect of its administration on hyperglycemia, to evaluate pancreatic histopathological changes in male rats with a type 2 diabetes mellitus model, and to determine the effective dose (ED₅₀). Fractionation of red shoot leaves was carried out using graded maceration, followed by phytochemical screening, specific and non-specific characterization, and determination of total phenolic and flavonoid contents. Experimental animals were induced with diabetes using a High Fat-Fructose Diet (HFFD) and streptozotocin (35 mg/kg body weight), then divided into treatment groups with doses of 50, 100, and 200 mg/kg body weight. The observed parameters included fasting blood glucose (FBG), AUC₀–₁₅, percentage reduction of blood glucose levels (%RBGL), and pancreatic histopathology. The results showed that the ethyl acetate fraction of red shoot leaves contained phenolics, flavonoids, and saponins and met the characterization requirements of the Indonesian Herbal Pharmacopoeia. Total flavonoid content was 50.13 ± 0.15 mg QE/g and total phenolic content was 65.87 ± 0.12 mg GAE/g. The fraction showed a dose-dependent increase in %RBGL, with the highest dose giving the optimal effect, an ED₅₀ of 185 mg/kg body weight, and improvement in islets of Langerhans structure. Based on these results, the ethyl acetate fraction of red shoot leaves has potential as an alternative for type 2 diabetes mellitus with good antidiabetic activity. Keywords: Ethyl acetate fraction, high fat diet–fructose diet, red shoot leaves, streptozotocin, type 2 diabetes mellitus.

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Detail Information

Series Title

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Call Number

T1971652026

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2026

Collation

xv, 102 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

615.321 07

Content Type

Text

Media Type

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Carrier Type

-

Edition

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Subject(s)

Tanaman obat
Prodi Ilmu Farmasi

Specific Detail Info

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Statement of Responsibility

TUTI

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