One of the freshwater bacteria that causes economic losses in freshwater fish farming is Aeromonas hydrophila because it can cause Motile Aeromonas Septicemia (MAS) disease. The use of lotus leaf extract as an antibacterial agent in Aeromonas hydrophila resulted in cell wall lysis and atrophy. This study purpose to determine the concentration of lotus leaf extract (Nymphaea pubescens L.) to inh…