Protein in feed will be hydrolyzed by rumen microbes into peptides and amino acids in the rumen fermentation process. The aim of this research was to study the total concentration of Volatile Fatty Acides (VFA) and N-Ammonia in Elephant grass incubated with rumen fluid adapted to NPN Ruminofrass in vitro. This research was carried out from September to November 2022 at the Nutrition and Animal …