Pain is a sign that something wrong in the body system such as tissue problem, also it is one of the most complaints when patient sees doctor. Pain can be treated with analgesic drug. One of plant which is widely used as an herbal medicine is Peronema canescens. The leaves of P. canescens has been reported contained betulinic acid, glycoside, β-sitosterol, stigmaterol and phytol. The aims of t…