One of the obstacles in tilapia cultivation is the Aeromonas hydrophila bacteria that causes Motile Aeromonas Septicemia (MAS) disease. This disease can be overcome by using natural ingredients. One of the natural ingredients that can be used to treat bacterial diseases in fish is the stem of brotowali (Tinospora crispa L.). The use of the stem of brotowali has been proven in vitro to inhibit A…