This study aims to determine the effect of fermented Polygonum barbatum application by supplementing Hymenachne acutigluma and Neptunia oleracea in rations on the in vivo digestibility of pampangan buffalo. This research was conducted from July to September 2022, at the Swamp Buffalo Development Center, Rambutan Village, Rambutan District, Banyuasin Regency, South Sumatra Province and Nutrient …