Completely Randomized Design (CRD) with 3 treatments and 3 replications was in this research. There were palm pressed fiber without amoniation (control), palm pressed fiber + 4% urea (RI), multilevel amoniation palm pressed fiber (R2). The parameters observed were microbial population such as amilolytic bacteria, cellulolytic, and preteolytic bacteria. The results showed that the treatment by m…