This research were done to study mix culture activity of various rumen fluid to overcome tannin in vitro. It was held for 2 (two) month at Animal Nutrition and Feed Laboratory Agriculture Faculty Sriwijaya University Compeletly randomized designed were done with 3 (three) proportion of rumen fluid from various ruminant (cattle, goat and buffalo). The treatments were P1 (25:50:25); P2 (25:25:50)…