One of the obstacles in tilapia cultivation is the Aeromonas hydrophila bacteria that causes Motile Aeromonas Septicemia (MAS) disease. This disease can be overcome by using natural ingredients. One of the natural ingredients that can be used to treat bacterial diseases in fish is the stem of brotowali (Tinospora crispa L.). The use of the stem of brotowali has been proven in vitro to inhibit A…
Secondary metabolite from ethyl acetate extract of Tinospora crispa stems has been isolated. The extraction was done by maceration, while the separation and purification of isolated compound was done by chromatographic technique. Structural determination of isolated compound was based on the analysis of spectroscopic data of ‘H NMR, l3C NMR, DEPT and compared to literature data. The isolated …
A secondary metabolites compound has been isolated from endophytic fungi Aspergillus sp2 is live in brotowali (Tinospora crispa L) stem plant’s. Isolated began by cultivation of Aspergillus sp2 fungi in 5 liters of Potato Dextrose Broth (PDB) media for eight weeks. The medium culture have been by partition using ethyl acetate and have been evaporated. The ethyl acetate concentrated extract ha…