A secondary metabolites compound has been isolated from endophytic fungi Aspergillus sp2 is live in brotowali (Tinospora crispa L) stem plant’s. Isolated began by cultivation of Aspergillus sp2 fungi in 5 liters of Potato Dextrose Broth (PDB) media for eight weeks. The medium culture have been by partition using ethyl acetate and have been evaporated. The ethyl acetate concentrated extract ha…
In Vivo Test of Methanol Extracts of Endophytic B\B Fungi Brotowali (Tinaspora crispa L.) has been tested as an antibacterials against Salmonella typhi and Shigella dysentriae by using Mice (Mus musculus L.). The research was previously conducted the isolation, culturitation and extraction until the methanol extract of endophytic B1B fungi was obtained which continued by doing the In Vivo test,…
Extracelluler major compound has been isolated from fungus Aspergillus sp 2 of Phaleria. Isolation started with the cultivation in 5 L PDB (Potato Dextrose Broth) medium for four weeks. Liquid medium was extracted with ethyl acetate and concentrated by rotary evaporator. Ethyl acetate extract was separated and purified by chromatographic techniques to obtain pure compound. The molecular structu…
The secondary metabolites compound have been isolated from endophytic fungi from root of Asam Kandis (Garcinia cowa). And than do it cultivation of Acremonium sp. fungi in 5 L of PDB's media ( Potato Dextrose Broth ) for 27 days. Liquid culture was extracted into the solvent ethyl acetate following by evaporation. Ethyl acetate extract was separated and purified by chromatography techniques in …
The secondary metabolite compound from endophytic fungi Fusarium sp of kunyit putih (Curcuma zedoaria (Berg) Roscoe) rhizome plant have been isolated. Isolation began with cultivation of Fusarium sp fungi in 8 L of PDB’s medium (Potato Dextrose Broth) until 21 days. The medium culture was extracted partition used ethyl acetate and then was evaporated. The ethyl acetate extract was separated a…
It has been isolated four endofhytic fungi from fruit Asam Kandis (iGarcinia Cowa) which is brown (CKLT), black and white fibers (SHP), Dark Green (HT), and black (HTM). These four fungi were identified as Aspergillus sp. Ethyl acetate extracts of four fiingi suggests that fungal extracts of Aspergillus sp (HT) has a major stain pattem. Aspergillus sp (HT) later in the cultivation medium in 3 L…
The endophytic microorganism is a form of bacteria or a fungi microorganism that form colonies interiororgans of plants, but does not harm the host plant. The secondary metabolites compound heve been isolated from endophytic fungi (Penicillium citrinum) living in symbiotic dill for malaria that is brotowali ( Tinospora crispa L ). Isolation begin with cultivation of Penicillium citrinum fungi i…
Spodoptera frugiperda (J. E. Smith) is a new pest in maize in Indonesia. S. frugiperda is very detrimental because it attacks maize plants in the larval stage. S. frugiperda attacks maize plants in the vegetative and generative phases which can cause damage to maize plants and even death. Endophytic fungi reside in plant tissues which can potentially be entomopathogenic fungi, which are a type …
Telah berhasil diisolasi senyawa metabolit sekunder dari jamur endofitik Aspergillus sp2 pada daun tumbuhan Sambiloto (Andrographis paniculata nees). Isolasi senyawa diawali dari kultivasi menggunakan media PDB (Potato Dextrose Agar) sebanyak 9 L dengan masa inkubasi selama 41 hari. Senyawa yang terdapat dalam medium kultur cair diekstraksi secara partisi menggunakan etil asetat, dievaporasi da…
Telah diisolasi satu senyawa golongan piranon dari jamur endofitik Aspergillus sp3 pada daun tumbuhan sambiloto (Andrographis paniculata Nees). Isolasi diawali dengan kultivasi jamur Aspergillus sp3 dalam 5 L media cair touge selama 4 minggu. Terhadap media dilakukan ekstraksi secara partisi menggunakan etil asetat dan dievaporasi. Ekstrak pekat diperoleh, dipisahkan, dan dimurnikan dengan tekn…
The secondary metabolites compound from endophytic fungi Penicillium sp living in kunyit putih rhizome plants (Curcuma zedoaria (Berg) Roscoe) have been isolated. Isolation began with cultivation of Penicilium sp fungi in 8 L of PDB’s media (Potato Dextrose Broth) until 24 days. The PDB’s media was extracted partially used ethyl acetate and then evaporated following by evaporation. The…
Telah diisolasi senyawa metabolit sekunder dari jamur endofitik Fusarium sp pada rimpang tumbuhan kunyit putih (Curcuma zedoaria (Berg) Roscoe). Isolasi diawali dengan kultivasi jamur Fusarium sp dalam 8 L medium PDB (Potato Dextrose Broth) selama 21 hari. Medium kultur diekstraksi secara partisi menggunakan etil asetat kemudian dievaporasi. Ekstrak pekat etil asetat yang diperoleh dipisahkan d…
ABSTRAK Telah dilakukan Uji In Vivo Ekstrak Metanol Jamur Endofitik B1B Tanaman Brotowali (Tinaspora Crispa L.) Sebagai Antibakteri terhadap Salmonella typhi dan Shigella dysentriae Menggunakan Mencit (Mus Musculus L.). Sebelumnya penelitian ini dilakukan isolasi, kulturisasi dan ekstraksi sehingga didapatkan Ekstrak Metanol Jamur Endofitik B1B yang selanjutnya dilakukan uji in vivo dengan men…