Protease is an enzyme capable of breaking peptide bonds in proteins into peptides and amino acids. Thermophilic bacteria from B. licheniformis are known as one of the microorganisms that produce protease capable of degrading keratin with high activity and under extreme conditions. In this study, protease enzyme production and purification were carried out from Bacillus licheniformis TS-8 isolat…
Penelitian ini dilaksanakan pada bulan November 2008 sampai dengan bulan Januari 2009 di Laboratorium Teknologi Hasil Perikanan Fakultas Pertanian Universitas Sriwijaya dan Laboratorium Bioproses Jurusan Teknik Kimia Fakultas Teknik Universitas Sriwijaya. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan enzim papain terhadap karakteristik saus remis. Rancangan yang digunakan adalah…
The aim of this research was to study the effect adding of phytase in broiler’s feed to its performance. This research was held for 7 weeks (May - June) in Laboratory Cages of Animal Nutrition and Feed Departement, Agriculture Faculty, University of Sriwijaya. A Completely randomized design with 6 treatment and 3 replication were used as follow ; P0 (basal ralion 100%), Pl(basal ration 100% +…
Telah dilakukan penelitian tentang “Studi Isolasi Enzim Proteolitik dari Getah Buah Pepaya (Carica papaya L) dan Penetapan Kualitasnya dengan Menggunakan Metode Penggumpalan Susu. Tujuan Penelitian ini adalah untuk memperoleh enzim proteolitik dari proses isolasinya dengan menggunakan filter papers 41 dan filter papers 42 dan untuk menetapkan kualitas enzim proteolitik dengan menggunakan meto…
Background: The liver is susceptible to damage due to exposure to free radicals, which trigger oxidative stress. This condition increases AST and ALT enzymes, which are markers of liver injury. This study used CCl₄-induced Wistar rats as a model of liver damage. Antioxidants are needed to prevent damage, and silymarin was used as a positive control. Purple sweet potato leaf extract contains f…
Amylase enzyme is an extracellular enzyme that can hydrolyze the internal bond of α 1,4-glucan in starch and is also one of the enzymes widely used in the industrial field. In industries that use enzymes, thermostable amylases with low substrate costs are the main choice because they can work at 60-120ºC and are usually produced by thermophilic bacteria. For certain purposes enzymes are often…
Diabetes mellitus is a disease that experiences an increase in the number of sufferers every year with treatment from synthetic ingredients that can cause harmful side effects to the body. Therefore, the discovery of a natural source of materials from Lansium domesticum Corr. that can inhibit the work of the enzyme α-glucosidase has emerged. Inhibition of the action of diabetes enzymes is one …
A Study Has Been Conducted To The Production Of Cellulase Enzyme From Aspergillus Niger Using Bagasse Substrate. Bagasse Is A Lignocellulosic Waste That Contains High Lignin So It Needs To Be Delignified To Remove The Lignin Content. Lignin Content Can Be Determined By The Chasson-Datta Method. The Delignification Process Succeeded In Reducing The Lignin Content By 71.84%, From 22.77% To 6.41%,…
The banana stem waste holds immense promise as a readily available and abundant source of lignocellulosic biomass, making it a compelling alternative for biofuel and biochemical applications. Therefore, this study focuses on investigating the impact of both time and substrate loading on the enzymatic hydrolysis of banana stem cellulose that has undergone alkaline microwave-assisted pre-treatmen…
Cellulase enzymes are a group of enzymes that break down cellulose by hydrolyzing β-1,4 glycosidic bonds into simpler forms such as glucose and cellobiose. This study aimed to confirm the cellulase-producing bacteria that have the highest cellulolytic activity and determine the optimal activity of pH, temperature, substrate concentration, and the influence of metal ions as cellulase enzyme act…
Extracellular azoreductase enzyme activity has been tested from Aeromonas jandaei bacteria. Extract of azoreductase enzyme from A. jandaei bacteria was obtained by centrifugation of liquid culture on NB media which was incubated for 2x24 hours at 37C in an incubator shaker. The results of enzyme fractionation with the highest specific activity were used to determine the optimum conditions fo…
Fungi Colletotrchum sp. is one of the fungi that can produce enzymes cellulase. The need for cellulase enzymes continues to increase because there are many of these enzymes used in various industrial fields. However, the production of these enzymes requires quite a long time and a large cost so that the optimization of process parameters production needs to be done, one of which is by optimizin…
Penelitian ini bertujuan untuk mengetahui nilai kecernaan silase serbuk pelepah sawit dan dedak yang disuplementasi vitamin B kompleks 2% dan mineral mix 1% secara in vitro meliputi bahan kering, bahan organik, dan serat kasar. Penelitian ini dilaksanakan dari bulan Agustus sampai dengan September 2022 di Laboratorium Nutrisi Pakan Ternak Program Studi Peternakan Fakultas Pertanian Universitas …
This study aims was to determine the digestibility value of palm frond powder which includes ADF, NDF, Hemicellulose, and Cellulose enriched with Vitamin B complex and mineral mix in Vitro. This research was carried out from August to September 2022 at the Animal Feed Nutrition Laboratory of the Animal Husbandry Study Program, Faculty of Agriculture, Sriwijaya University. This study used palm f…
LUSI TRI UTAMI, Pengaruh Waktu Perendaman Dengan Enzim Papain Terhadap Karakteristik Fisik Dan Kimia Daging Keong mas (Dibimbing oleh AGUS SUPRIADI). Penelitian ini bertujuan untuk mengetahui waktu optimum perendaman daging keong mas di dalam enzim papain untuk mendapatkan karakteristik fisik dan kimia terbaik. Penelitian ini menggunakan 3 perlakuan pengamatan perendaman daging keong mas di dal…
Penelitian ini bertujuan untuk mengeksplorasi bakteri penghasil enzim kitosanase diambil dari tumbuhan yang ada di perairan rawa Sakatiga, Indralaya dan Mengukur aktivitas enzim kitosanase yang dihasilkan oleh bakteri dari tumbuhan rawa. Penelitian ini dilaksanakan pada bulan Oktober sampai Januari 2009 di Laboratorium Teknologi Hasil Perikanan dan Laboratorium Mikrobiologi Budidaya Perairan. F…
The aim of this research was to study the effect addition of phytase in basic diets contain rice brand and com to the performance of Arabian chicken eggshell in the first production. This research was held on Balai Pembibitan Ternak Unggul (BPTU) Sapi Dwiguna dan Ayam Petelur Sembawa for six weeks from January - February 2009. This research using completely randomized design (RAL) with six ....…
Tujuan dari penelitian ini adalah membuat hidrolisat protein dari ikan patin (Pangasius pangasius) secara enzimatis menggunakan enzim papain dan menganalisis aktivitas antioksidan dari hidrolisat protein yang dihasilkan. Penelitian dilaksanakan dari bulan Desember 2013 sampai Januari 2014 di Laboratorium Teknologi Hasil Perikanan Program Studi Teknologi Hasil Perikanan, Laboratorium Kimia dan M…
Penelitian ini bertujuan untuk mengeksplorasi bakteri penghasil enzim kitosanase diambil dari saluran pencernaan ikan di perairan rawa Sakatiga, Indralaya, Ogan Ilir, Sumatera Selatan. Penelitian ini dilaksanakan pada bulan Oktober 2009 sampai Januari 2010 di Laboratorium Teknologi Hasil Perikanan dan Laboratorium Mikrobiologi Budidaya Perairan, Fakultas Pertanian Universitas Sriwijaya dan Labo…
Vitex pinnata merupakan salah satu tumbuhan tropis dan sub-tropis yang dilaporkan memiliki berbagai bioaktivitas. Pengujian aktivitas penghambatan enzim α-glukosidase dan kandungan senyawa dari batang tumbuhan V. pinnata belum pernah dilaporkan sebelumnya. Berdasarkan hal tersebut penelitian ini dilakukan untuk mengisolasi senyawa metabolit sekunder dan uji aktivitas antidiabetes menggunakan e…
Extracellular azoreductase enzyme activity test has been carried out from the Pseudomonas stutzeri. P. stutzeri is bacteria bacteria that have the ability to decolorize azo dyes against methyl red dyes.This study aimed to examine the activity of the extracellular azoreductase enzyme from P. stutzeri. The azoreductase enzyme extract from P. Stutzeri was obtained by centrifugation of the liquid c…
Ludwigia octovalvis termasuk salah satu jenis tumbuhan tropis yang berpotensi sebagai tumbuhan obat. Bioaktivitasnya sebagai antidiabetes dalam menghambat enzim α-glukosidase dari bagian buah tumbuhan ini belum pernah dilaporkan begitu juga dengan metabolit sekundernya. Penelitian ini bertujuan untuk mengisolasi senyawa metabolit sekunder dan penentuan aktivitas antidiabetes dalam menghambat e…
ABSTRACT : Cellulase enzymes are enzymes that play a role in breaking down complex polymers of cellulose into simple monomers of glucose. Cellulase enzymes can be produced by cellulolytic fungi isolated from various types of substrates, one of which is acacia litter which has high cellulose content. Cellulase enzymes are enzymes that are widely developed in various industrial activities. To mee…
The isolation of triterpenoid from ethyl acetate fraction of Ludiwigia peruviana leaves and α-glucosidase enzyme inhibition test from extract, fraction and the isolated compound had been conducted. The isolation process was conducted by maceration using methanol as the solvent, then continued with liquid-liquid partition using n-hexane and ethyl acetate. Ethyl acetate fraction was separated an…
Ludwigia peruviana is one of the 82 species of Ludwigia that grows in the tropical and sub-tropical. The extract of L. adscendens has been reported as antidiabetic activity. Meanwhile, the antidiabetic activity and the chemical compound isolated from L. peruviana has never been reported yet. Generally, the same genus have the similar compound. Therefore, the research was conducted to isolated t…
Telah dilakukan penelitian tentang skrining dan optimasi pertumbuhan bakteri termofilik pendegradasi limbah bulu ayam dari isolat sumber air panas Tanjung Sakti Lahat di Laboratorium Biokimia Fakultas MIPA Universitas Sriwijaya. Skrining terhadap 10 jenis isolat bakteri diperoleh 7 isolat yang dapat menghasilkan enzim keratinase. Melalui pengukuran diameter zona bening dipilih dua isolat yang m…
Telah dilakukan penentuan kondisi optimum aktivitas enzim xilanase termostabil dari bakteri Anoxybacillus rupiensis. Pengamatan dilakukan terhadap pertumbuhan Anoxybacillus rupiensis selama 24 jam, setiap 2 jam diukur OD (Optical dencity). Ekstrak kasar enzim diperoleh dengan cara sentrifugasi pada kecepatan 3000 rpm. Uji aktivitas dengan metode DNS dan penentuan kadar protein dengan metod…