Red galangal (Alpinia purpurata) is a species of the Zingiberaceae family that has potential as a source of bioactive compounds. The main objectives of this study were to identify secondary metabolites from red galangal rhizomes (A. purpurata) and to determine their antioxidant and antibacterial activities. Red galangal rhizomes were extracted and then separated to obtain fractions. The extract…
Lumpur minyak bumi tergolong dalam limbah bahan berbahaya dan beracun (B3) yang dapat mencemari dan merusak lingkungan. Penelitian ini bertujuan untuk mempelajari karakterisasi sampel lumpur minyak bumi yang didapat dari PT Pertamina EP Prabumulih Field. Penelitian ini juga mempelajari pengaruh suhu dan waktu ekstraksi terhadap hasil minyak dengan pelarut toluena kombinasi surfaktan Triton X-10…
This study utilizes TKKS, which is rich in cellulose and has the potential to be processed into glucose. The conversion of cellulose into glucose is carried out through enzymatic hydrolysis. The kinetics of hydrolysis is determined using the Michaelis-Menten equation and transformed into the Lineweaver-Burk method to obtain Vmax and Km parameters. The research findings show that enzyme activity…
This study utilizes TKKS, which is rich in cellulose and has the potential to be processed into glucose. The conversion of cellulose into glucose is carried out through enzymatic hydrolysis. The kinetics of hydrolysis is determined using the Michaelis-Menten equation and transformed into the Lineweaver-Burk method to obtain Vmax and Km parameters. The research findings show that enzyme activity…
The banana stem waste holds immense promise as a readily available and abundant source of lignocellulosic biomass, making it a compelling alternative for biofuel and biochemical applications. Therefore, this study focuses on investigating the impact of both time and substrate loading on the enzymatic hydrolysis of banana stem cellulose that has undergone alkaline microwave-assisted pre-treatmen…
Semprawang (D. ochreata) is plant belongs to Dilleniaceae family that has been used by Musi tribe as a cure for scabies. The information about biology activity from this plant is very limited. The aims research was to screen of antioxidant compound from steam bark of D. ochreata. The research was started with extraction by maceration method using a solvent of increasing polarity (n-hexane, ethy…
: Penelitian ini telah dilakukan penentuan pengaruh ion logam (CaCl2; FeCl3; dan MnSO4) dengan variasi konsentrasi 1mM dan 5 mM terhadap aktivitas enzim selulase yang dihasilkan E. cloacae dan K. quasipneumoniae serta menentukan kondisi pH dan suhu optimum enzim selulase tersebut. Penentuan pH, suhu dan pengaruh ion logam terhadap enzim selulase dengan metode Miller dilakukan pada fraksi 40 –…
Research on the activity of extracellular azoreductase enzymes from Bacillus tropicuss BD 01 has been carried out. Extract of azoreductase enzyme from bacteria B. tropicuss BD 01 was produced by centrifugation process of bacterial liquid culture at 3000 rpm and 4°C. The crude extract of the enzyme was then fractionated with ammonium sulfate and dialyzed. The results of the enzyme fraction with…
Termites are pests that can cause damage to various types of wood, buildings and plants. Termites can digest cellulose because their intestines contain cellulolytic bacteria. Cellulolytic bacteria from termite digestion have been isolated, two of which are Enterobacter Clocoae and Klebsiella Pneumoniae. This study aimed to study the effect of pH and temperature on cellulase enzyme activity isol…
Alkaloids have been isolated from the bark of tembesu (Fragraea fragrans Roxb). This study complements the report on the alkaloid content of the tembesu bark. The antibacterial activity test was carried out against Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Tembesu bark was extracted by maceration using diethyl ether and fractionated with ethyl acetate, separation and purificati…
Skrining bakteri proteolitik terhadap 15 isolat bakteri termofilik pada media susu skim telah berhasil dilakukan. Isolat bakteri terpilih kemudian ditentukan waktu optimum inkubasi dengan perhitungan jumlah sel pada kurva pertumbuhan dengan menggunakan hemasitometer dan uji aktivitas enzim dengan metode Bergmeyer pada media susu skim dan tepung bulu ayam. Ekstrak kasar enzim difraksinasi menggu…
Enzim amilase merupakan salah satu jenis enzim yang luas pemanfaatannya dalam industri. Pada industri dibutuhkan amilase yang dapat bekerja pada temperatur tinggi dan produksi amilase dengan biaya substrat yang murah. Oleh karena itu dibutuhkan enzim termostabil yang dapat dihasilkan dari bakteri termofilik dan media alternatif yang dapat menghasilkan amilase dengan baik serta lebih hemat biaya…
Penelitian ini telah dilakukan skrining terhadap 15 bakteri termofilik isolat Sumber Air Panas Tanjung Sakti Lahat sebagai penghasil selulase, menentukan waktu pertumbuhan bakteri dalam memproduksi enzim selulase pada media sekam padi dan CMC dengan metode hemasitometer dan Miller, serta mengetahui karakteristik enzim selulase yang dihasilkan yang meliputi karakterisasi terhadap suhu (30; 40; 5…
Enzim azoreduktase diperoleh dari bakteri Bacillus tropicus BD 01. Produksi enzim azoreduktase ditingkatkan dengan cara penambahan inducer. Inducer yang ditambahkan yaitu CaCl2, CaCO3, dan metil merah dengan konsentrasi 1 mM pada saat inokulasi dan kulturisasi. Bakteri Bacillus tropicus BD 01 yang telah diinokulasi dan dikulturkan pada media cair nutrient broth selanjutnya di sentrifugasi denga…
A bio-briquette product innovation had prepared from a mixture of cassava skin (KS) and Rubber Seed Shell (TBK) using H2SO4 activator. The combination of KS and TBK raw materials used is 70:30% with a total weight of 8 grams, with various variables including carbonization temperature, acid compound concentration and length of chemical activation time. Variables used at carbonization temperature…
Biji sacha inchi (Plukenetia volubilis), yang kemudian diolah menjadi minyak sacha inchi merupakan tanaman yang kaya akan kandungan asam lemak seperti asam linoleat dan linolenat, juga oleat, palmitat dan stearat dalam jumlah kecil. Kandungan-kandungan asam lemak pada minyak sacha inchi ini dapat meningkatkan pertumbuhan bakteri yang menguntungkan bagi manusia, salah satunya bakteri probiotik B…
Research on the prebiotic properties of semende coffee fortified with inulin powder and its test against Lactobacillus bulgaricus FNCC-0041 has been carried out. The aim of the study was to make prebiotic coffee from inulin. Inulin as a prebiotic can increase the growth of probiotic bacteria in the digestive tract. Before adding inulin, coffee was fermented with Saccharomyces cerevisiae and com…
Borax is a chemical compound derived from boron (B), which is widely used as an antifungal agent, wood preservative, and antiseptic in cosmetics. Boron mostly differs in chemical properties, but in biological activity by forming complexes with biomolecules one of which is pyridoxine. This research aims to study the complexation between boron and pyridoxine with variations in time, temperature a…
Stefanot Ungu (Mansoa alliacea) termasuk salah satu tumbuhan yang berasal dari genus Bignoniaceae yang memiliki bioaktivitas sebagai antihiperkolestromia, antifungi, dan antioksidan dari ekstrak dan fraksi. Pengujian penghambatan enzim α-glukosidase belum pernah dilaporkan. Penelitian ini bertujuan untuk mengisolasi senyawa kimia dan pengujian aktivitas antidiabetes dari ekstrak metanol, fraks…
Daun rukam (Flacourtia rukam) telah dimanfaatkan masyarakat sebagai obat tradisional untuk radang kelopak mata yang berkaitan dengan aktivitas antiinflamasi. Senyawasenyawa yang memberikan aktivitas antiinflamasi umumnya memberikan aktivitas antioksidan. Penelitian ini bertujuan untuk melakukan skrining aktivitas antioksidan pada fraksi etil asetat daun tumbuhan rukam. Penelitian diawali dengan…
Enzim azoreduktase diperoleh dari bakteri Aeromonas jendaei. Produksi enzim azoreduktase ditingkatkan dengan cara penambahan induser. Induser yang ditambahkan yaitu CaCl2, CaCO3 dan metil merah dengan konsentrasi 1 mM pada saat inokulasi dan kulturisasi. Bakteri A. jendaei yang telah diinokulasi dan dikulturkan pada media cair nutrient broth dengan dan tanpa penambahan induser, selanjutnya di s…
Propionibacterium acne (P. acne) is a group of bacteria that causes acne. P. acne lived by consuming chemical compounds from sweat glands and fat on the skin face. Fluids in acne containspus, red blood cell lysis component and cytokine which is produced by cell’s of body defences. P. acne bacteria hides in that fluids and can proliferate. Most of free molecule of clindamycin can not reach P. …
Steroid compound from n-hexane fraction of asam kandis (Garcinia Cowa) stem has been isolated. Isolation begins with the extraction by maceration using increasing polarity (n-hexane, ethyl acetate and methanol). The separation and purification performed by chromatographic techniques. Determining the structure of the isolated compound based on the analysis of spectroscopic data include UV, IR an…
The stigmast-en-3-on have been isolated from nonpolar fraction of methanol extract of betel nut leaves (Areca catechu L.)- The extraction was done by maceration. Separation and purification was conducted by chromatographic techniques. The isolated compound was yellow solids with melting point 97-99°C. The structure of this compound was determined base on spectroscopic data including UV, 1R, 13…
Conversion of fructose to 5-hydroxymethylfurfural and conversion of 5-hydroxymethylfurfural to levulinic acid using hydrochioric acid and zeolite in water as a medium in various reaction conversion temperature has been carried out. Product of reaction conversion was monitored by Thin Layer Chromatography (TLC) and analized quantitatively using GC and GC-MS. The results showed that fructose can …
Conversion of glucose and fructose into levulinic acid has been carried out using natural zeolite and bentonite as catalysts. Natural zeolite and bentonite were characterized using X-Ray Diffractometer and FT-IR spectrophotometer before catalytic process. Reaction of glucose and fructose in water medium at 80°C using natural zeolite and bentonit until 10 hours did not produce levulinic acid. T…
Research about ability chitin and membrane of chitin had been done for the adsorption of lead ion. Chitin was isolated from prawn shells PT Lolamina Palembang. Characterized chitin determined by measuring deacetylation degree, water content, and ash content. Membrane of chitin as an adsorbent prepared by 0.01 g chitin and mixture of solvent DMA, NMP, LiCl and PVC as a support matrix. Characteri…